A Dithiothreitol-Sensitive Tetrameric Protease from Spinach Thylakoids Has Polyphenol Oxidase Activity
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چکیده
منابع مشابه
C-Terminal Propeptide of BKA has a Protease Sensitive Structure Without any Inhibitory Effect on BKA
In our previous study, we compared the two α-amylase enzymes from Bacillus sp.KR8104, BKA∆(N44) and BKA∆(N44C193) which is the secreted form of it. The results indicated that the presence of 193 amino acids propeptide in the C-terminal of BKA∆(N44) changed its enzymatic parameters like an uncompetitive inhibitor in comparison to BKA∆(N44C193). In the present study, we cloned the DNA sequence of...
متن کاملDirect Electrochemistry of Polyphenol Oxidase
The electrochemistry of banana tissues on a carbon paste electrode modified with multi-walled carbon nanotubes (MWCNTs) is presented. Cyclic voltammetry is applied to investigate the direct electrochemistry of banana tissues i.e. a source of polyphenol oxidase (PPO). A redox couple with an anodic and counterpart cathodic peak is obtained. The influence of various parameters such as pH,...
متن کاملPolyphenol oxidase activity expression in Ralstonia solanacearum.
Sequencing of the genome of Ralstonia solanacearum revealed several genes that putatively code for polyphenol oxidases (PPOs). To study the actual expression of these genes, we looked for and detected all kinds of PPO activities, including laccase, cresolase, and catechol oxidase activities, in cellular extracts of this microorganism. The conditions for the PPO assays were optimized for the phe...
متن کاملUncoupling of Spinach Thylakoids by Gramine
Gramine and related alkaloids isolated from fodder plants in the Gramineae, Leguminoseae and other plant families [1] are responsible for the toxicity observed in ruminants grazing on these plants [2, 3]. Recently, these alkaloids were reported as toxic to aphids feeding on graminaceous crops [4]. Toxicity of gramine has been related to the known physiological effects of tryptamine related comp...
متن کاملA Study on Polyphenol Oxidase Activity During Seed Germination
KOCAQALISKAN I., DEMIR Y. & KABAR, K. 1995. A study on polyphenol oxidase activity during seed germination. Phyton (Horn, Austria) 35 (1): 37-43. English with German summary. In this work, polphenol oxidase (PPO) activities, when used four different substrates (dopa,catechol,caffeic acid, tyrosine) oxidizing activities, during seed germination of six different plants were studied in the embryos...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 1997
ISSN: 0032-0781,1471-9053
DOI: 10.1093/oxfordjournals.pcp.a029150